Mitra was born to Shibnarayan Mitra in Kolkata, British India. He passed from Hare School and entered into the Presidency College but due to poor economic condition he had to left study and started living in his maternal house at Barasat (presently North 24 Parganas district). His elder brother was a notable doctor, Nabinkrishna Mitra.
Mitra involved with progressive education movement and several social reformative actions in Bengal. In 1847 he established a private girls school in Barasat with the help of his brother Nabinkrishna and educationist Peary Charan Sarkar. This was the first school for the girls of aristocratic Hindu families established by any Indian. Initially it was started with only two girls. Kuntibala, daughter of Nabinkrishna is one of them. Although such activities was strongly opposed by Hindu Zaminders and the then conservative society but Ishwar Chandra Vidyasagar and John Elliot Drinkwater Bethune supported Mitra's enormous effort for women education in Bengal. Latter the school was renamed as Kalikrishna Girls' High School. Even Bethune got inspiration for establishment Bethune School in 1949, when he went there for inspection as President of the Council of Education. Mitra organised an agricultural firm of 150 Bighas for scientific farming, plantation and research in Barasat. He brought modern equipment from England for this purpose. He also contributed to spread out for Homeopathy medication.
- Bama Chikitsa
- Garhasthyobabostha O Shishu Chikitsa
- Pashu Chikitsa
- Vol II, Anjali Basu (2004). Sansad Bangali Charitabhidhan. Kolkata: Sahitya Sansad. p. 78. ISBN 81-86806-99-7.
- Chapter 15, Subal Chandra Mitra. "Isvar Chandra Vidyasagar, a story of his life and work". en.wikisource.org. Retrieved April 21, 2018.
- "Barasat Government College". Retrieved April 21, 2018.
- Chiranjit Roy. "Madanmohan Tarkalankar and Women Education in the First Half of 19th Century Bengal" (PDF). Retrieved April 21, 2018.
- Ishvarchandra Vidyasagar. "Hindu Widow Marriage". Retrieved April 21, 2018.
- Bagal, Jogesh C., History of The Bethune School and College in the Bethune School and College Centenary Volume, 1849–1949.
- Projit Bihari Mukharji. "Nationalizing the Body: The Medical Market, Print and Daktari Medicine". Retrieved April 21, 2018.
- Mitra, Kalikrishna. "Garhasthya-byabastha O Shishu Chikitsa". Retrieved April 21, 2018.